Data_Sheet_1_Chemical Variation of Chenpi (Citrus Peels) and Corresponding Correlated Bioactive Compounds by LC-MS Metabolomics and Multibioassay Analysis.DOCX

The peel of Citrus reticulata “Chachi” (CP) possesses various health-promoting benefits and is not only one of the most famous Chinese herbal medicine, but also an ingredient in fermented foods. In the present study, the effects of storage years (1-, 3-, 4-, 5-, 6-, and 11-years) on the chemical profiling and potential bioactive compounds of CP were compared by metabolomics and in vitro bioactivity analysis. With the increase of storage time, the content of hesperidin significantly decreased, but nobiletin, 3,5,6,7,8,3′,4′-heptamethoxyflavone, and tangeretin were increased. Meanwhile, the antioxidant activity of CP was enhanced. Phenolic acids, flavonol glycosides, fatty acids, and alkyl glycosides were marker compounds that were responsible for distinguishing the storage time of CP. Correlation analysis suggested that some polyphenols including quercetin-glucoside, quinic acid, trihydroxydimethoxyflavone, and rutin were potential antioxidant compounds in CP. The dichloromethane and n-butanol fractions showed the better antioxidant capacity and inhibitory effects on glucose-hydrolysis enzymes. They mainly contained ferulic acid, nobiletin, 3,5,6,7,8,3′,4′-heptamethoxyflavone, kaempferol, and hesperidin.

广陈皮（Citrus reticulata 'Chachi'，简称CP）具备多种健康益处，不仅是最知名的中药材之一，同时也是发酵食品的配料。本研究采用代谢组学结合体外生物活性分析方法，比较了不同陈化年限（1、3、4、5、6及11年）的广陈皮的化学组分特征与潜在生物活性成分。随着陈化时间延长，橙皮苷（hesperidin）含量显著降低，而川陈皮素（nobiletin）、3,5,6,7,8,3′,4′-七甲氧基黄酮（3,5,6,7,8,3′,4′-heptamethoxyflavone）及橘皮素（tangeretin）的含量则呈上升趋势。与此同时，广陈皮的抗氧化活性亦随之提升。酚酸、黄酮醇苷、脂肪酸及烷基糖苷是区分广陈皮陈化年限的标志性成分。相关性分析结果显示，槲皮素葡萄糖苷（quercetin-glucoside）、奎宁酸（quinic acid）、三羟基二甲氧基黄酮（trihydroxydimethoxyflavone）及芦丁（rutin）等多酚类物质是广陈皮中潜在的抗氧化活性成分。二氯甲烷萃取相和正丁醇萃取相表现出更优的抗氧化能力及对葡萄糖水解酶的抑制活性，其主要成分为阿魏酸（ferulic acid）、川陈皮素、3,5,6,7,8,3′,4′-七甲氧基黄酮、山奈酚及橙皮苷。