Expression profiling analysis of an inducible ELF3-expressing and control human bile duct cells [inducible ELF3]

An immortalized normal epithelial cell line of common bile duct origin (HBDEC2 cells) was established. An inducible ELF3-expressing was generated by infection with the retrovial vector pRetroX-TetOne-Puro-ELF3-flag, expressing ELF3 fused to a flag-tag. We performed a genome-wide expression profiling analysis of an inducible ELF3-expressing cells and control cells. Three independent samples were performed.

成功构建了一株来源于胆总管的永生化正常上皮细胞系（HBDEC2细胞）。通过逆转录病毒载体pRetroX-TetOne-Puro-ELF3-flag感染，构建了可诱导表达ELF3的细胞系，该载体表达与Flag标签（flag-tag）融合的ELF3蛋白。我们对可诱导表达ELF3的细胞系及对照细胞系开展了全基因组表达谱分析，共设置三组独立生物学重复样本。