Differentially expressed genes after miRNA or siRNA transfection in human cancer cell lines II

To identify differentially expressed genes by anti cancer treatments (microRNAs or siRNAs) in human cancer, several cell lines (pancreatic cancer, esophageal cancer, bladder cancer, prostate cancer, renal cell carcinoma and lung squamous cell carcinoma) were subjected to Agilent whole genome microarrays. Human cell lines (Panc-1, sw1990, TE8, TE9, A549, MRC-5, BOY, T24, PC3, C4-2, 786-O, A-498 and EBC-1) were treated with miRNAs (miR-375, miR-29a, miR-26a, miR-145-5p, miR-145-3p, miR-218, miR-320a), siRNAs (si-MMP11, si-LAMP1, si-LOXL2, si-PLOD2, si-UHRF1, and si-FOXM1).

为鉴定人类癌症中抗癌治疗（微小RNA（microRNA，miRNA）或小干扰RNA（small interfering RNA，siRNA））诱导的差异表达基因，本研究将多株涵盖胰腺癌、食管癌、膀胱癌、前列腺癌、肾细胞癌及肺鳞状细胞癌来源的细胞系进行安捷伦全基因组微阵列（Agilent whole genome microarrays）检测。所用人类细胞系包括Panc-1、sw1990、TE8、TE9、A549、MRC-5、BOY、T24、PC3、C4-2、786-O、A-498及EBC-1，分别采用miR-375、miR-29a、miR-26a、miR-145-5p、miR-145-3p、miR-218、miR-320a等miRNA，以及si-MMP11、si-LAMP1、si-LOXL2、si-PLOD2、si-UHRF1、si-FOXM1等siRNA进行处理。