Transcriptome-wide Association study identifies putative Elicitors/Suppressor of Puccinia graminis f. sp. tritici that Modulate Barley rpg4-mediated Stem Rust Resistance

Transcriptomic profiling was done on barley samples incoulated with different Puccinia graminis f. sp. tritici (Pgt) isolates of varying virulence profile on barley stem rust resistance gene rpg4/5 and Rpg1. Several differentally expressed host and pathogen genes were identified while comparing gene expression profiles between group of samples inoculated with isoaltes of varying virulence. Several variants with genes of pathogen were idnetified that are possibly associated with virulence to rpg4/5. Suceptible barley cv. Harrignton were treated with 24 Pgt isolates, indivdually and samples were collected 5 days post inoculation.

本研究对大麦样本开展转录组分析（Transcriptomic profiling），这些样本分别接种了毒力特征各异的小麦秆锈菌（Puccinia graminis f. sp. tritici，以下简称Pgt）分离物，此类分离物针对大麦秆锈病抗性基因rpg4/5与Rpg1呈现出差异化的毒力表现。通过对比不同毒力分离物接种组的基因表达谱，本研究鉴定出多个宿主与病原菌的差异表达基因；同时还鉴定出多个病原菌基因变异体，这些变异体可能与针对rpg4/5的毒力相关。此外，本研究以感病大麦品种Harrignton为材料，分别接种24株Pgt分离物，并于接种后5天采集样本。