Metabolomics Workbench ST000775 - GNPS - Michigan Biomarkers for Refractory Depression (Bluebird) metabolomics pilot study

The goal of the project is discovery of clinical useful biomarkers for treatment-resistant depression. All participants were undergoing electroconvulsive therapy (ECT) for treatment-resistant depression (major depressive disorder or bipolar disorder) in 2012-2014. All subjects were Caucasian except BB060, who was Asian. Samples were collected from each subject at baseline just before the first ECT treatment (Time Point T0) and approximately 3 weeks later just before the tenth ECT treatment (Time Point T2). Whole blood was drawn in the morning in a fasted state (at least 7 hours) through an intravenous catheter or butterfly needle into 6-mL EDTA tubes (Lavender Top, Becton Dickinson Vacutainer K2EDTA additive blood collection tube, part #367863) and transported to the processing lab at the Michigan Clinical Research Unit within 30 minutes. Plasma was isolated by centrifugation for 10 minutes at 2000G at 4C. Plasma from multiple tubes was pooled and aliquotted in 200 uL volumes into 2-mL screw-cap cryovials and frozen at -80C.

本项目的研究目标为发掘难治性抑郁症（treatment-resistant depression）中具有临床应用价值的生物标志物。所有受试者均于2012-2014年间因难治性抑郁症（包括重度抑郁症（major depressive disorder）或双相障碍（bipolar disorder））接受电抽搐治疗（electroconvulsive therapy, ECT）。除受试者BB060为亚裔外，其余所有受试者均为高加索人种。分别于首次电抽搐治疗前的基线时刻（时间点T0），以及约3周后第十次电抽搐治疗前（时间点T2），从每位受试者处采集样本。采血于清晨空腹状态（空腹时长至少7小时）下进行，通过静脉导管或蝶形针采集血液至6mL EDTA采血管（该采血管为Becton Dickinson Vacutainer K2EDTA添加剂采血管，货号367863，紫色管帽），并在30分钟内转运至密歇根临床研究中心的样本处理实验室。采用4℃、2000G离心10分钟的方法分离血浆；将多管采集得到的血浆混合后，按200μL每份的体积分装至2mL螺口冻存管中，最终于-80℃条件下冷冻保存。