Comparative Genome-Wide Profiling of Transposon and Retrovirus Integration Sites in Primary Human CD4+ T Cells. Homo sapiens

We mapped Sleeping Beauty (SB) and piggyBac (PB) insertions in primary human CD4+ T cells and compared their insertion profiles with those of the MLV retrovirus and the HIV lentivirus with respect to proximity to genes, TSSs, CpG islands, DNase I hypersensitive sites, repetitive elements, chromatin marks and transcriptional status of genes. Overall design: Examination of two transposon systems in one cell type.

本研究对原代人CD4阳性T细胞中的睡美人（Sleeping Beauty, SB）转座子与piggyBac（PB）转座子的插入位点进行了定位，并将二者的插入图谱与MLV逆转录病毒、HIV慢病毒的插入图谱进行对比，分析维度涵盖基因邻近区域、转录起始位点（Transcription Start Sites, TSSs）、CpG岛、DNase I超敏位点、重复序列元件、染色质修饰标记以及基因的转录状态。实验设计概述：在单一细胞类型中对两种转座子系统进行检测分析。