Metabolic prioritizations of Polar cod (Boreogadus saida) at ambient and elevated water temperatures

Two groups (n = 8, n = 5) of Polar cod (Boreogadus saida) were acclimated to both 0 and 6°C. The smaller groups were used to investigate specific dynamic action (SDA) (treatment "digestion"). Each fish of the larger groups was used for exercise tests in an unfed and fed state (treatment "exercise" and "exercise & digestion", respectively) at their respective acclimation temperatures as well as in a fed state acutely exposed to an intermediate temperature (3°C). SDA measurements lasted for 18 and 9 days at 0 and 6°C, identical to the food-deprivation period of B. saida in the exercise approach. Feeding was conducted in the common tank. Accordingly, daily rations reflect the mean food consumption per treatment. Each fish was allowed to feed for at least 2h before the start of the experiments.

将两组北极鳕（Boreogadus saida，n分别为8和5）驯化至0°C和6°C两种温度条件下。较小的一组用于研究特殊动力作用（specific dynamic action, SDA）（处理组“消化”）。较大一组的每条鱼均在其各自驯化温度下，分别于未进食和进食状态（对应处理组“运动”和“运动&消化”）进行运动测试；此外，还在进食状态下急性暴露于中间温度（3°C）时进行测试。0°C和6°C条件下的SDA测量持续时间分别为18天和9天，与运动测试中北极鳕的禁食期长度一致。喂食操作在公共水槽中进行，因此每日投喂量反映了各处理组的平均食物消耗量。每条鱼在实验开始前均被允许进食至少2小时。