Expression data from M0505 (with and without PAX2) murine ovarian surface epithelial cell lines. Mus musculus

Our lab established the M0505 cell line from the ovarian surface epithelium (OSE) of FVB/N mice in May 2005 in order to study OSE biology. This cell line was infected with PAX2 to study the biological consequences of PAX2 expression in normal mOSE cells We used microarrays to try to determine the downstream targets of PAX2 in mOSE (M0505) cells to gain information that may lead to a greater understanding of its oncongenic potential. Overall design: Two biological replicates of M0505+WPI, M0505+PAX2 and M0505+PAX2+Cre cells were collected for RNA extraction and subesequent hybridization on the Affymetrix MoGene-1_0-st-v1 platform.

本实验室于2005年5月从FVB/N小鼠的卵巢表面上皮（Ovarian Surface Epithelium, OSE）中构建了M0505细胞系，用于开展卵巢表面上皮的生物学研究。为探究正常小鼠卵巢表面上皮细胞中PAX2表达的生物学效应，本研究将PAX2基因转染至该细胞系。为获取有助于深入解析PAX2致癌潜能的相关信息，我们借助基因芯片技术，旨在鉴定小鼠卵巢表面上皮（M0505）细胞中PAX2的下游靶基因。

整体设计：
本实验收集了M0505+WPI、M0505+PAX2及M0505+PAX2+Cre三组细胞的两份生物学重复样本，完成RNA提取后，在Affymetrix MoGene-1_0-st-v1芯片平台上进行杂交实验。