Dataset from Mouse 6 for Sit & Goard: Distributed and Retinotopically Asymmetric Processing of Coherent Motion in Mouse Visual Cortex

Accompanying dataset for Sit KK, Goard MJ. Distributed and Retinotopically Asymmetric Processing of Coherent Motion in Mouse Visual Cortex.<br><br>This dataset contains the data for mouse 6 / 10<br>Mouse SKKS047<br><br>See all data in collection here: https://doi.org/10.35092/yhjc.c.5018363<br>These datasets contain calcium imaging data taken from L2/3 of the primary visual cortex in mice. Mice were head fixed and passively watching a random dot kinematogram stimulus. The stimulus data vector for processing these data are also included. The accompanying code to analyze these data can be found at: https://github.com/kevinksit/CoherentMotionProject<br>Data are either in preprocessed DFF matrices, which represent the change in neural activity compared to a baseline or raw images from the microscope. All data are processed with MATLAB 2018b.<br>The purpose of these data is to understand the organization of neural activity in response to coherent motion in the murine visual cortex.<br>The manuscript associated with these data is Sit KK, Goard MJ. Distributed and Retinotopically Asymmetric Processing of Coherent Motion in Mouse Visual Cortex.<br>ABSTRACTPerception of visual motion is important for a range of ethological behaviors in mammals. In primates, specific visual cortical regions are specialized for processing of coherent visual motion. However, whether mouse visual cortex has a similar organization remains unclear, despite powerful genetic tools available for measuring population neural activity. Here, we use widefield and 2-photon calcium imaging of transgenic mice to measure mesoscale and cellular responses to coherent motion. Imaging of primary visual cortex (V1) and higher visual areas (HVAs) during presentation of natural movies and random dot kinematograms (RDKs) reveals varied responsiveness to coherent motion, with stronger responses in dorsal stream areas compared to ventral stream areas. Moreover, there is considerable anisotropy within visual areas, such that neurons representing the lower visual field are more responsive to coherent motion. These results indicate that processing of visual motion in mouse cortex is distributed heterogeneously both across and within visual areas.

本数据集配套于Sit KK与Goard MJ发表的学术论文《小鼠视觉皮层中相干运动的分布式与视拓扑不对称加工》。

本数据集包含第6/10号实验小鼠的数据，对应小鼠编号SKKS047。

可通过以下链接查看该合集内的全部数据：https://doi.org/10.35092/yhjc.c.5018363

本数据集包含取自小鼠初级视觉皮层（primary visual cortex, V1）第2/3层（L2/3）的钙成像（calcium imaging）数据。实验中小鼠头部固定，并被动观看随机点运动图（random dot kinematogram）刺激范式，同时包含用于该数据分析的刺激数据向量。

用于解析本数据集的配套代码可通过以下链接获取：https://github.com/kevinksit/CoherentMotionProject

数据集包含两类数据：一类为预处理后的ΔF/F矩阵（DFF matrices），代表神经活动相对于基线的变化量；另一类为显微镜采集的原始图像。所有数据均通过MATLAB 2018b完成处理。

本数据集的研究目标为解析小鼠视觉皮层中响应相干运动的神经活动组织模式。

本数据集关联的学术论文为Sit KK与Goard MJ发表的《小鼠视觉皮层中相干运动的分布式与视拓扑不对称加工》。

摘要
视觉运动感知对哺乳动物的多种本能行为至关重要。在灵长类动物中，特定视觉皮层区域会特化负责相干视觉运动的加工。尽管当前已拥有可用于记录群体神经活动的高效遗传学工具，但小鼠视觉皮层是否存在类似的功能组织模式仍未明确。
本研究通过对转基因小鼠进行宽场成像与双光子钙成像（2-photon calcium imaging），记录其对相干运动的介观尺度与单细胞水平响应。在呈现自然影片与随机点运动图（RDKs）的过程中，对初级视觉皮层（V1）与高级视觉皮层区域（HVAs）的成像结果显示，不同脑区对相干运动的响应存在差异：背侧通路区域的响应强度显著高于腹侧通路区域。此外，单个视觉皮层区域内部也存在显著各向异性：代表下视野的神经元对相干运动的响应更强。上述结果表明，小鼠皮层对视觉运动的加工在不同视觉皮层区域间以及单个区域内部均呈现异质性分布式特征。