Transcriptome-wide gene expression of the heart of neonatal rats with polygenic cardiac hypetrophy

Identification of genes differentially expressed between the neonatal heart of a genetic rat model of cardiac hypertrophy (the Hypertrophic Heart Rat, HHR) and the control (the Normal Heart Rat, NHR) using Affymetrix GeneChip® Rat Gene 1.0 ST Arrays. Neonatal (day 2) HHR and age-matched NHR were killed with an overdose of pentobarbitone (Lethobarb). The heart was immediately removed, and the ventricles were dissected from the atriums. The tissues were first preserved in liquid nitrogen and later transferred to a –80C freezer. Microarrays (one per sample, with no pooling) were performed on 8 neonatal HHR (4 male, 4 female) and 8 neonatal NHR (4 male, 4 female). After extraction of RNA, cRNA was prepared and arrays performed using Affymetrix GeneChip® Rat Gene 1.0 ST Arrays performed at the Ramaciotti Gene Function Analysis facility, University of New South Wales in Sydney, Australia.

本研究采用Affymetrix GeneChip®大鼠基因1.0 ST芯片（Affymetrix GeneChip® Rat Gene 1.0 ST Arrays），鉴定心肌肥大遗传大鼠模型——肥厚型心脏大鼠（Hypertrophic Heart Rat, HHR）与对照品系正常心脏大鼠（Normal Heart Rat, NHR）新生心脏组织间的差异表达基因。选取出生后第2天的新生HHR与同周龄匹配的NHR，通过过量注射戊巴比妥（Lethobarb）实施安乐死。迅速取出心脏，将心室与心房分离。分离得到的组织样品先置于液氮中临时保存，随后转移至-80℃超低温冰箱长期储存。本研究共纳入8只新生HHR（4雄4雌）与8只新生NHR（4雄4雌），每个样品单独进行基因芯片检测，未进行样品混合处理。完成总RNA提取后，制备互补RNA（cRNA），并使用Affymetrix GeneChip®大鼠基因1.0 ST芯片完成芯片杂交检测，实验工作于澳大利亚悉尼新南威尔士大学拉马乔蒂基因功能分析中心开展。