CENH3-ChIP-seq of Chamaelirium luteum to determine the size and sequence composition of the large centromeres.

To determine the size and sequence composition of the exceptionally large centromeres in the genome of monocentric Chamaelirium luteum, we performed CENH3-ChIPseq using the customized species-specific CENH3 antibody. We mixed the chromatins of C. luteum and Secale cereal (inbred line Lo7) to dilute the highly abundant centromeric Chama satellite repeats (9.3%) in the C. luteum genome before immunoprecipitation. Overall design: The CENH3-ChIP-Seq experiment was performed using a mixture of chromatin isolated from flower tissue of C. luteum and leaf tissue of Secale cereale inbred line Lo7. The normalized CENH-ChIP-Seq signal track of the average of read counts in ChIP over input in a genome-wide 1 kb windows.

为解析单着丝粒黄藜芦（Chamaelirium luteum）基因组中异常巨大的着丝粒的大小与序列组成，我们使用定制的物种特异性CENH3抗体开展了CENH3染色质免疫共沉淀测序（CENH3-ChIP-seq）实验。在免疫沉淀前，我们将黄藜芦与黑麦（Secale cereale，近交系Lo7）的染色质混合，以稀释黄藜芦基因组中占比9.3%的高丰度Chama卫星重复序列。
实验整体设计：本研究采用分别分离自黄藜芦花组织与黑麦近交系Lo7叶组织的混合染色质开展CENH3-ChIP-seq实验。全基因组1kb窗口范围内，ChIP样本读数计数与输入对照样本读数计数的平均值，构成了标准化的CENH3-ChIP-seq信号轨迹。