Short-term insulin intensive therapy decreases MCP-1 and NF-κB expression of peripheral blood monocyte and the serum MCP-1 concentration in newlydiagnosed type 2 diabetics

ABSTRACT Objective To observe the effect of short-term insulin intensive treatment on the monocyte chemoattractant protein-1 (MCP-1) as well as on the nuclear factor-kappa B (NF-κB) expression of peripheral blood monocyte. This is also in addition to observing the serum MCP-1 level in newlydiagnosed type 2 diabetic patients and probing its anti-inflammation effects. Subjects and methods Twenty newly-diagnosed type 2 diabetic patients were treated with an insulin intensive treatment for 2 weeks. MCP-1 and NF-κB expression on the monocyte surface were measured with flow cytometry, the serum MCP-1 level was measured by enzyme linked immunosorbent assay (ELISA) during pretreatment and post-treatment. Results After 2 weeks of the treatment, MCP-1 and NF-κB protein expression of peripheral blood monocyte and serum MCP-1 levels decreased significantly compared with those of pre-treatment, which were (0.50 ± 0.18)% vs (0.89 ± 0.26)% (12.22 ± 2.80)% vs (15.53 ± 2.49)% and (44.53 ± 3.97) pg/mL vs (49.53 ± 3.47) pg/mL, respectively (P < 0.01). The MCP-1 expression on monocyte surface had a significant positive relationship with serum MCP-1 levels (r = 0.47, P < 0.01). Conclusions Short-term insulin intensive therapy plays a role in alleviating the increased inflammation reaction in type 2 diabetics.

摘要
目的 观察短期胰岛素强化治疗对初诊2型糖尿病患者外周血单核细胞趋化蛋白-1（monocyte chemoattractant protein-1, MCP-1）及核因子-κB（nuclear factor-kappa B, NF-κB）表达的影响，同时检测患者血清MCP-1水平，探讨其抗炎作用。
研究对象与方法 选取20例初诊2型糖尿病患者，给予为期2周的胰岛素强化治疗。采用流式细胞术检测单核细胞表面MCP-1及NF-κB的表达水平，分别于治疗前及治疗后采用酶联免疫吸附试验（enzyme linked immunosorbent assay, ELISA）检测血清MCP-1水平。
结果 治疗2周后，患者外周血单核细胞MCP-1、NF-κB蛋白表达水平及血清MCP-1水平均较治疗前显著降低，分别为(0.50±0.18)% vs (0.89±0.26)%、(12.22±2.80)% vs (15.53±2.49)%及(44.53±3.97)pg/mL vs (49.53±3.47)pg/mL（P<0.01）。单核细胞表面MCP-1表达与血清MCP-1水平呈显著正相关（r=0.47，P<0.01）。
结论 短期胰岛素强化治疗可缓解2型糖尿病患者体内的炎症反应，发挥抗炎功效。