A high-quality Transcriptome Reference for the Tasmanian devil.

This project provides a new transcriptome assembly for the Tasmanian devil (Sarcophilus harrisii). Frozen tissue RNA extractions were performed by Max Stammnitz (Department of Veterinary Medicine, University of Cambridge, UK).
Data citation
For use of these resources, please cite: The evolution of two transmissible cancers in Tasmanian devils (Stammnitz et al. 2023, Science 380:6642) Comment
RNA for this species transcriptome annotation was sourced from 33 Tasmanian devils between 2003 and 2017, and respectively sequenced on the equivalent of 1/3 of an Illumina HiSeq 4000 lane, yielding about 100 Million 75bp PE reads per sample. Mostly in biological duplicates, the featured tissues are: axillary nerve, bone marrow, brachial plexus nerve, brain stem, cerebellum, cerebrum, heart, liver, lung, optic nerve, ovary, pancreas, sciatic nerve, skin, spleen, testis, thyroid and trigeminal nerve.

本项目为袋獾（Sarcophilus harrisii）提供了一套全新的转录组组装结果。冷冻组织RNA提取工作由英国剑桥大学兽医学院的马克·施塔姆尼茨（Max Stammnitz）完成。
数据引用
若使用本研究资源，请引用以下文献：《袋獾体内两种可传播癌症的演化》（Stammnitz等，2023，《科学》，380卷：6642期）
备注
用于该物种转录组注释的RNA样本采集自2003年至2017年间的33只袋獾，每个样本的测序数据量相当于Illumina HiSeq 4000测序仪单泳道的1/3，单样本可获得约1亿条75bp双端（PE）测序读段。本次测序的组织样本大多设置了生物学重复，涵盖的组织类型包括：腋神经、骨髓、臂丛神经、脑干、小脑、大脑、心脏、肝脏、肺脏、视神经、卵巢、胰腺、坐骨神经、皮肤、脾脏、睾丸、甲状腺以及三叉神经。