A longitudinal and transancestral analysis of DNA methylation patterns and disease activity in lupus patients

Epigenetic dysregulation is implicated in the pathogenesis of lupus. We performed a longitudinal analysis to assess changes in DNA methylation in neutrophils collected from 54 lupus patients over 4 years of follow up and across disease activity levels using 229 patient samples. Illumina Infinium MethylationEPIC array of genomic DNA from neutrophils extracted from whole blood from 54 systemic lupus erythematosus (SLE) patients over time for a total of 229 timepoints.

表观遗传调控异常与狼疮的发病机制存在密切关联。本研究开展纵向分析，利用229份患者样本，对54名狼疮患者4年随访期间采集的中性粒细胞DNA甲基化变化，以及不同疾病活动度下的甲基化改变进行评估。本研究采用Illumina Infinium MethylationEPIC芯片，对54名系统性红斑狼疮（systemic lupus erythematosus, SLE）患者不同时间点采集的全血中提取的中性粒细胞基因组DNA进行甲基化检测，共计229个时间点样本。