Single-cell mRNA profiling reveals changes in solute carrier expression and a metabolic switch during zebrafish pronephros development

Developing organisms need to adapt to environmental variations as well as to rapid changes in substrate availability and energy demands imposed by fast-growing tissues and organs. Little is known about the adjustments that kidneys undergo in response to these challenges. We performed single-cell RNA sequencing of zebrafish pronephric duct cells to understand how the developing kidney responds to changes in filtered substrates and intrinsic energy requirements. Overall design: Single cell RNA sequencing was performed on pronephric cells from 1, 2 and 3-day old zebrafish larvae.

发育中的生物体需适应环境变化，同时也要应对快速生长的组织与器官所引发的底物可利用性改变及能量需求波动。目前学界对肾脏应对此类挑战的调节机制尚缺乏充分认知。为探究发育中的肾脏如何响应滤过底物变化与内在能量需求，我们对斑马鱼前肾管（pronephric duct）细胞开展了单细胞RNA测序（single-cell RNA sequencing）。整体实验设计：我们对1日龄、2日龄及3日龄斑马鱼幼体的前肾细胞开展了单细胞RNA测序。