Proteomic analysis of integrin adhesion complex disassembly induced by nocodazole

To explore the dynamics of integrin adhesion complex disassembly, isolated adhesion complexes from U2OS cells were subjected to mass spectrometry based proteomic analysis. U2OS cells were allowed to spread on FN, treated with nocodazole for 4 hours to disrupt microtubules. To coordinate microtubule-induced integrin adhesion complex disassembly nocodazole was subsequently washed out and adhesion complexes isolated at 5, 10 and 15 minutes during this process.

为探究整合素黏着复合体（integrin adhesion complex）的解离动力学，研究人员从U2OS细胞中分离得到黏着复合体，并对其开展基于质谱的蛋白质组学分析。研究人员将U2OS细胞铺展于纤连蛋白（FN）表面，经诺考达唑（nocodazole）处理4小时以破坏微管；为调控微管诱导的整合素黏着复合体解离过程，随后洗脱去除诺考达唑，并于该过程的第5、10和15分钟时分批次分离黏着复合体。