Gossypium raimondii strain:D5-2, D5-4, D5-31, and D5-53 Genome sequencing. Gossypium raimondii strain:D5-2, D5-4, D5-31, and D5-53

Plant material was grown and gathered from greenhouses at Brigham Young University (D5-2, D5-31, A1-155, A2-34, A2-1011) and Iowa State University (D5-4, D5-53, A2-4, A1-73). DNA was extracted from 4 accessions of G. raimondii (D5-2, D5-4, D5-31, D5-53), 2 accessions of G. herbaceum (A1-73, A1-155), and 3 accessions of G. arboreum (A2-4, A2-34, A2-1011) using a Qiagen DNeasy plant kit. DNA libraries were prepared with the Illumina TruSeq V3 kit and sequenced by BGI, producing 100 bp paired-end reads. Approximately 40x genomic coverage was obtained for each library (Table 1).

本研究涉及的植物材料均种植并采集自杨百翰大学（Brigham Young University）与爱荷华州立大学（Iowa State University）的温室，其中杨百翰大学提供的材料编号为D5-2、D5-31、A1-155、A2-34、A2-1011，爱荷华州立大学提供的材料编号为D5-4、D5-53、A2-4、A1-73。随后，采用Qiagen DNeasy植物试剂盒，分别从4份雷蒙德氏棉（G. raimondii）种质材料（D5-2、D5-4、D5-31、D5-53）、2份草棉（G. herbaceum）种质材料（A1-73、A1-155）以及3份树棉（G. arboreum）种质材料中提取基因组DNA。使用Illumina TruSeq V3试剂盒构建DNA测序文库后，由BGI完成测序，产出100 bp双端读段（paired-end reads）。每个文库均获得约40倍的基因组测序覆盖度，相关信息详见表1。