Distalised kidney organoids. Distalised kidney organoids

Distalised organoid samples at Day 14 and Day 21 of culture. Day 21 cultures have RSPO1 addition either at Day 7 or Day 14 of culture. Overall design: Single cell RNA-seq generated on a 10x Chromium genomics platform from iPSC-derived human kidney organoids. Each sample represents 6 pooled organoids derived from a single well of a transwell plate, generated from individual iPSC-derived reporter lines and harvested at either Day 14 (D14) or Day 21 (D21) of the differentiation protocol.

本数据集包含培养至第14天和第21天的远端化类器官样本，其中培养至第21天的样本均在培养第7天或第14天添加了RSPO1。整体实验设计：本数据集为基于10x Chromium基因组学平台（10x Chromium genomics platform）获取的诱导多能干细胞（induced pluripotent stem cell，iPSC）来源的人肾脏类器官单细胞RNA测序数据。每一份样本均由来自Transwell板单个孔的6个混合类器官构成，这些类器官源自独立的iPSC来源报告基因细胞系，并在分化方案的第14天（D14）或第21天（D21）收获。