Data_Sheet_3_Comprehensive Mapping of the Cell Response to Borrelia bavariensis in the Brain Microvascular Endothelial Cells in vitro Using RNA-Seq.XLSX

Borrelia bavariensis can invade the central nervous system (CNS) by crossing the blood-brain barrier (BBB). It is predicted that B. bavariensis evokes numerous signaling cascades in the human brain microvascular endothelial cells (hBMECs) and exploits them to traverse across the BBB. The complete picture of signaling events in hBMECs induced by B. bavariensis remains uncovered. Using RNA sequencing, we mapped 11,398 genes and identified 295 differentially expressed genes (DEGs, 251 upregulated genes and 44 downregulated genes) in B. bavariensis challenged hBMECs. The results obtained from RNA-seq were validated with qPCR. Gene ontology analysis revealed the participation of DEGs in a number of biological processes like cell communication, organization of the extracellular matrix, vesicle-mediated transport, cell response triggered by pattern recognition receptors, antigen processing via MHC class I, cellular stress, metabolism, signal transduction, etc. The expression of several non-protein coding genes was also evoked. In this manuscript, we discuss in detail the correlation between several signaling cascades elicited and the translocation of BBB by B. bavariensis. The data revealed here may contribute to a better understanding of the mechanisms employed by B. bavariensis to cross the BBB.

巴伐利亚疏螺旋体（Borrelia bavariensis）可通过穿越血脑屏障（blood-brain barrier, BBB）侵袭中枢神经系统（central nervous system, CNS）。据推测，巴伐利亚疏螺旋体可在人脑微血管内皮细胞（human brain microvascular endothelial cells, hBMECs）中引发多条信号级联反应，并利用这些反应完成血脑屏障的穿越。目前，巴伐利亚疏螺旋体诱导hBMECs内信号事件的完整图景仍未被阐明。本研究通过RNA测序（RNA sequencing）技术，对巴伐利亚疏螺旋体侵染的hBMECs进行了基因表达谱分析，共绘制得到11398个基因的表达图谱，并鉴定出295个差异表达基因（DEGs），其中上调基因251个、下调基因44个。RNA测序所得结果通过定量聚合酶链反应（qPCR）得到了验证。基因本体（gene ontology, GO）分析显示，上述差异表达基因参与了多种生物学过程，包括细胞通讯、细胞外基质组织、囊泡介导的运输、模式识别受体触发的细胞应答、通过主要组织相容性复合体I类（MHC class I）介导的抗原加工、细胞应激、代谢以及信号转导等。本研究同时发现，多个非蛋白编码基因的表达亦被诱导上调。本文详细探讨了巴伐利亚疏螺旋体引发的多条信号级联反应与该菌穿越血脑屏障过程之间的关联。本研究所得数据可为进一步解析巴伐利亚疏螺旋体穿越血脑屏障的致病机制提供重要的理论参考。