monocyte reprogramming by oxLDL

We have performed analyses of murine primary bone marrow derived monocytes challenged with either PBS or oxLDL. oxLDL selectively enhances growth and adhesion potential of monocytes. Purified bone marrow monocytes were treated with PBS or 20 microgram/ml oxLDL for a five day period, and cells were harvested for scRNAseq analyses. Overall design: WT murine monocytes were treated with either PBS, 10 microg/ml oxLDL for a five day period

本研究针对经磷酸盐缓冲液（PBS）或氧化低密度脂蛋白（oxLDL）刺激的小鼠原代骨髓来源单核细胞开展了分析。实验表明，oxLDL可选择性增强单核细胞的增殖能力与黏附潜能。本实验将纯化后的骨髓单核细胞以PBS或20 μg/mL的oxLDL处理5天，随后收集细胞用于单细胞RNA测序（scRNAseq）分析。实验设计：将野生型（WT）小鼠原代骨髓单核细胞分别以PBS、10 μg/mL的oxLDL处理5天。