Table_5_Quantitative Trait Loci Mapping and Marker Identification for Low Salinity Tolerance Trait in the Swimming Crab (Portunus trituberculatus).xlsx

Low salinity is one of the most important abiotic factors that directly affect the abundance of the swimming crab, Portunus trituberculatus. Quantitative trait loci (QTL) mapping could be helpful in identifying the markers and genes involved in low salinity tolerance. In this study, two QTLs of low salt tolerance were mapped on linkage group 17 (LG17, 2.6–5.2 cM) based on a high-density linkage map. Ninety-five markers related to low salinity tolerance were identified via association analysis, and seventy-nine low salt-related candidate genes (including ammonium transport, aldehyde dehydrogenase, and glucosyltransferase) were screened from draft genome of the species via these markers. This represents the first report of QTL mapping for low salinity tolerance in the swimming crab, which may be useful to elucidate salinity adaptation mechanisms.

低盐度是直接影响三疣梭子蟹（Portunus trituberculatus）种群丰度的关键非生物因子之一。数量性状位点（Quantitative trait loci，QTL）定位技术可用于鉴定与低盐耐受相关的分子标记及功能基因。本研究基于高密度连锁图谱，在第17连锁群（LG17，2.6–5.2 cM）上定位到2个与低盐耐受相关的QTL。通过关联分析，本研究共鉴定出95个与低盐耐受相关的分子标记，并依托这些标记从该物种的草图基因组中筛选得到79个低盐耐受相关候选基因，涵盖铵转运蛋白、醛脱氢酶与糖基转移酶等功能类别。本研究首次完成三疣梭子蟹低盐耐受相关QTL的定位研究，相关成果可为阐明该物种的盐度适应机制提供重要参考。