VD3-VDR axis regulates the homeostasis and function of alveolar macrophage [RNA-Seq]

Purpose: To understand the regulation of gene expression of alveolar macrophages by VD3-VDR axis Fresh isolated WT alveolar macropahges (AM) were treated with or without 50 nM Vitamin D3 in vitro for 24hrs. Fresh AMs were isolated from WT and VDR knockout mice in 4 and 10 weeks old. RNA was isolated and purified by Qiagen Rneasy micro kit and applied to RNAseq.

本数据集的研究目的为阐明VD3-VDR轴对肺泡巨噬细胞基因表达的调控作用。体外实验中，将新鲜分离的野生型（Wild Type, WT）肺泡巨噬细胞（alveolar macrophages, AM）分别以50 nM维生素D3（Vitamin D3）处理与不做处理，孵育24小时。此外，分别从4周龄和10周龄的野生型及维生素D受体（Vitamin D Receptor, VDR）敲除小鼠体内分离新鲜肺泡巨噬细胞。随后使用Qiagen Rneasy micro试剂盒提取并纯化总RNA，开展RNA测序（RNAseq）实验。