Seq [organism=Pectobacterium carotovorum subsp. carotovorum] [clone=Bgl3] gene, Complete cds. Bgl3-a clone encoded Beta-glucosidase from Pectobacterium carotovorum subsp. carotovorum

The bacterium Pectobacterium carotovorum subsp. carotovorum (Pcc) was used as a DNA source for Bgl3 amplification, a gene encodes for a β-glucosidase. Primers’ design was carried out using an existing Pcc gene-sequences, for genomic DNA amplification and Escherichia coli clone transformation using pTAC-MAT-2 as an expression vector. Molecular and biochemical studies were conducted for the assimilated clone product alongside three other clones' isolated using the same source, which could provide evidence for successful transformation of a lignocellulosic biomass oligomers into biofuel related precursors. *the clone's DNA was sequenced via Molecular Cloning Laboratories ex-facility (MCLAB, San Francisco, CA, USA).>SeqATGAAATGGCTTACTTCACTGACTATAGCAATTGGACTTGCCTGTAATCCGGCGTTTGCGCAGGAGCTGACGTCTGCGCCAGCCTCAATATCGCCTTCACACCAGCAACGTGATGCGTTTGTCACGGATCTGCTGAAGAAAATGACGCTGGAAGAGAAAATCGGCCAGCTCCGATTAATCAGCGTCGGGACGGATAACCCGAAAGAAGCCATTCGGGAAATGATCAGAAACGGCCAGGTTGGCGCGATTTTTAATACAGTGACCCGTCCAGATATCCGTGAGATGCAAGATCAGGTTATGCAGCTCAGTCGCCTGAAAATTCCGCTGTTTTTCGCCTACGATGTGGTGCATGGCCAGCGCACGATTTTCCCTATCGCGCTGGGGCTAGCTTCCAGTCGGGATATGGACGCGATTGAGAAAAGTGCGCGCGTAGCGGCGTATGAAGCGACGGAAGACGGTCTGAACATGACCTGGGCGCCGATGGTGGATATCACCCGCGATCCGCGCTGGGGCCGCGTTTCAGAAGGCTTCGGTGAAGATACTTGGTTGACCAGTAAAATTGCCGGTGTGGTAGTCAAATCCTTTCAGGGCGATGACGTAACCGGACGCCACTCGCTGATGACCAGCGTAAAACACTACGCGCTCTACGGTGCGGTGGAAGGCGGACGTGACTATAACACCGTGGATATGAGCCCTCAGCGCATGTTCCAAGACTATATGCCGCCTTACAAAGCGGCGATTGACGCGGGCAGCAGCGGCGTGATGGTTGCGTTGAACTCAATCAACGGTACGCCAGCCACCTCAAATAGCTGGTTGCTGAAAGAGGTTCTGCGCGATCAGTGGAATTTCAAAGGCATCACCATTACCGATCACGGCGCGATTAAAGAGCTGATTAAGCACGGTGTGGCCAGCGATCCGCGTGATGCATCGCGTCTGGCGATTAAATCCGGTATCGGCATGAGCATGAGCGACGAGTATTTCGTGCGCTATCTGCCTGAGCTGGTGAAAAGTGGGGCGGTGAGCATGCAGGAGATTGACGATGCCAGCCGTCAGGTGCTGAACGTGAAATACGATATGGGGCTGTTTGAAGATCCGTATCGTCATTTGGGGCCAGTCGGTTCCGATCCGGTAGATACTAACGCGGAAAGTCGCTTACACCGTCTGGACGCGCGTGACGTGGCGCGTAAGAGTCTGGTGCTGTTGAAAAACCGCCTACAAACGCTGCCGTTGAAGAAAGAAGGCACGATTGCCGTTGTGGGGCCATTGGCTGACAGCCAGCGCGATACGATGGGAAGCTGGTCTGCCGCAGGCGTCACGAAACAGACGATTACCGTTTATCAGGGCCTGAAGAATGCCGTCGGCGATAAAGCCACCATTCTTTATGCTAAAGGTGCCAACGTCAGTAATCACAAAGGCATTATCGATTTCCTGAATCAGTATGAAGATGCCGTTCAGGTGGATAAACGTCCTCCGCAGGTCATGATCGATGAAGCCGTAGCCGCAACGAAGAAGGCGGATGTGGTGGTTGCGTCGTTGGTGAAGCGGCCGGGATGGCGCATGAAGCATCTAGCCGTTCGAACATCGACCTGCCGCAAGGCCAGCGTGACCTGATTGCGGCGCTGAAAGCCACAGGTAAACCGCTGGTTCTGGTGCTGATGAACGGACGCCCTCTGGCGCTAGTGCGTGAAGATCAGCAAGCCGATGCGTTACTGGAAACCTGGTTCAGCGGTACGGAAGGCGGCAATGCGATCGCCGATGTGCTGTTTGGCGACTATAACCCATCTGGCAAACTGCCGATGTCCTTCCCACGCTCCGTCGGCCAGATCCCGATTTATTACAATCACCTGCCGTCAGGTCGTCCTTACACGCCGGAAAACCCAGGCAAATATACGTCTCACTACTACGATGAAGCTAATGGGCCGCTCTATCCGTTTGGTTACGGCCTGAGCTACACCACGTTTAGTGTGTCAGACGTGCGTTTATCCAGCCAGACGATGAAGCGTAACGGGACGATCAACGCCAGCGTCACGGTGAAAAACACCGGCAACCGCGCAGGGGAAACGGTCGTACAGCTGTATCTCCATGATGTGGTGGCTTCCATCAGCCGTCCGCTGAAAGAGCTGCGTGGTTTTGAGAAAGTGATGCTACAGCCGGGAGAATCCCGCACGGTCACCTTCACGCTCGATCAGGACGCACTGAAGTTCTACAATGCCCGCATGCAGCAGGTGGTGGAACCTGGCAAATTCGACGTGATGATTGGTCTGGATTCACAGCGCGTGAAGAGCGATAGCTTCACCTTGCTATAA

研究以胡萝卜软腐果胶杆菌胡萝卜亚种（Pectobacterium carotovorum subsp. carotovorum，简称Pcc）作为DNA来源，开展编码β-葡萄糖苷酶（β-glucosidase）的Bgl3基因扩增实验。引物设计基于已公开的Pcc基因序列，用于基因组DNA扩增，并以pTAC-MAT-2作为表达载体，完成大肠杆菌（Escherichia coli）克隆转化。针对该克隆产物以及另外3株从同一来源分离得到的克隆株，开展了分子与生化特性研究，该研究可为木质纤维素生物质寡聚物成功转化为生物燃料相关前体物质提供实验依据。*本克隆的DNA由美国加利福尼亚州旧金山分子克隆实验室外包设施（MCLAB）完成测序。SeqATGAAATGGCTTACTTCACTGACTATAGCAATTGGACTTGCCTGTAATCCGGCGTTTGCGCAGGAGCTGACGTCTGCGCCAGCCTCAATATCGCCTTCACACCAGCAACGTGATGCGTTTGTCACGGATCTGCTGAAGAAAATGACGCTGGAAGAGAAAATCGGCCAGCTCCGATTAATCAGCGTCGGGACGGATAACCCGAAAGAAGCCATTCGGGAAATGATCAGAAACGGCCAGGTTGGCGCGATTTTTAATACAGTGACCCGTCCAGATATCCGTGAGATGCAAGATCAGGTTATGCAGCTCAGTCGCCTGAAAATTCCGCTGTTTTTCGCCTACGATGTGGTGCATGGCCAGCGCACGATTTTCCCTATCGCGCTGGGGCTAGCTTCCAGTCGGGATATGGACGCGATTGAGAAAAGTGCGCGCGTAGCGGCGTATGAAGCGACGGAAGACGGTCTGAACATGACCTGGGCGCCGATGGTGGATATCACCCGCGATCCGCGCTGGGGCCGCGTTTCAGAAGGCTTCGGTGAAGATACTTGGTTGACCAGTAAAATTGCCGGTGTGGTAGTCAAATCCTTTCAGGGCGATGACGTAACCGGACGCCACTCGCTGATGACCAGCGTAAAACACTACGCGCTCTACGGTGCGGTGGAAGGCGGACGTGACTATAACACCGTGGATATGAGCCCTCAGCGCATGTTCCAAGACTATATGCCGCCTTACAAAGCGGCGATTGACGCGGGCAGCAGCGGCGTGATGGTTGCGTTGAACTCAATCAACGGTACGCCAGCCACCTCAAATAGCTGGTTGCTGAAAGAGGTTCTGCGCGATCAGTGGAATTTCAAAGGCATCACCATTACCGATCACGGCGCGATTAAAGAGCTGATTAAGCACGGTGTGGCCAGCGATCCGCGTGATGCATCGCGTCTGGCGATTAAATCCGGTATCGGCATGAGCATGAGCGACGAGTATTTCGTGCGCTATCTGCCTGAGCTGGTGAAAAGTGGGGCGGTGAGCATGCAGGAGATTGACGATGCCAGCCGTCAGGTGCTGAACGTGAAATACGATATGGGGCTGTTTGAAGATCCGTATCGTCATTTGGGGCCAGTCGGTTCCGATCCGGTAGATACTAACGCGGAAAGTCGCTTACACCGTCTGGACGCGCGTGACGTGGCGCGTAAGAGTCTGGTGCTGTTGAAAAACCGCCTACAAACGCTGCCGTTGAAGAAAGAAGGCACGATTGCCGTTGTGGGGCCATTGGCTGACAGCCAGCGCGATACGATGGGAAGCTGGTCTGCCGCAGGCGTCACGAAACAGACGATTACCGTTTATCAGGGCCTGAAGAATGCCGTCGGCGATAAAGCCACCATTCTTTATGCTAAAGGTGCCAACGTCAGTAATCACAAAGGCATTATCGATTTCCTGAATCAGTATGAAGATGCCGTTCAGGTGGATAAACGTCCTCCGCAGGTCATGATCGATGAAGCCGTAGCCGCAACGAAGAAGGCGGATGTGGTGGTTGCGTCGTTGGTGAAGCGGCCGGGATGGCGCATGAAGCATCTAGCCGTTCGAACATCGACCTGCCGCAAGGCCAGCGTGACCTGATTGCGGCGCTGAAAGCCACAGGTAAACCGCTGGTTCTGGTGCTGATGAACGGACGCCCTCTGGCGCTAGTGCGTGAAGATCAGCAAGCCGATGCGTTACTGGAAACCTGGTTCAGCGGTACGGAAGGCGGCAATGCGATCGCCGATGTGCTGTTTGGCGACTATAACCCATCTGGCAAACTGCCGATGTCCTTCCCACGCTCCGTCGGCCAGATCCCGATTTATTACAATCACCTGCCGTCAGGTCGTCCTTACACGCCGGAAAACCCAGGCAAATATACGTCTCACTACTACGATGAAGCTAATGGGCCGCTCTATCCGTTTGGTTACGGCCTGAGCTACACCACGTTTAGTGTGTCAGACGTGCGTTTATCCAGCCAGACGATGAAGCGTAACGGGACGATCAACGCCAGCGTCACGGTGAAAAACACCGGCAACCGCGCAGGGGAAACGGTCGTACAGCTGTATCTCCATGATGTGGTGGCTTCCATCAGCCGTCCGCTGAAAGAGCTGCGTGGTTTTGAGAAAGTGATGCTACAGCCGGGAGAATCCCGCACGGTCACCTTCACGCTCGATCAGGACGCACTGAAGTTCTACAATGCCCGCATGCAGCAGGTGGTGGAACCTGGCAAATTCGACGTGATGATTGGTCTGGATTCACAGCGCGTGAAGAGCGATAGCTTCACCTTGCTATAA