LHRH Receptor Monoclonal Antibody 4F3B10 Induces Apoptosis of HeLa Cells

In this study, based on microarray hybridization and the detections of RTqPCR and Western Blotting, we have shown that HeLa cells treated with LHRH receptor monoclonal antibody 4F3B10 increased protein expressions in Fas signaling pathway, which provided a novel route for the gene therapy of HeLa cells. The effects of antagonist 4F3B10 on HeLa cell proliferation, cell cycle and apoptosis were detected by CCK-8 assay and flow cytometry. Gene expression profiles were utilized to screen the differentially expressed genes in HeLa cells with the effects of antagonist 4F3B10 vs. the control, and analyze the relation among the differentially expressed genes by bioinformatics analysis.

本研究基于基因芯片杂交（microarray hybridization）、实时定量聚合酶链反应（RTqPCR）及蛋白质印迹（Western Blotting）实验，证实经促黄体生成素释放激素（LHRH）受体单克隆抗体4F3B10处理的海拉细胞（HeLa），其Fas信号通路（Fas signaling pathway）的蛋白表达水平显著上调，该发现为海拉细胞的基因治疗提供了全新途径。本研究采用细胞计数试剂盒-8（CCK-8）检测法与流式细胞术，检测了拮抗剂4F3B10对海拉细胞增殖、细胞周期及凋亡的影响。通过基因表达谱筛选得到拮抗剂4F3B10处理组与对照组海拉细胞的差异表达基因，并通过生物信息学分析阐明了该类差异表达基因间的相互关联。