Analysis of gene expression in co-culture of PBMCs with colon cancer cell lines by High-Throughput Sequencing

(Objectives) The goal of this study is to investigate paracrine effect of peripheral blood mononuclear cells (PBMCs) on colon cancer cell lines. (Method) Transcription profiles of PBMCs (control) and PBMCs co-cultured with colon cancer cell lines (treatment) were generated by deep sequencing, in triplicate for SW480 and duplicate for HT29, using Illumina HiSeq 4000. (Results and conclusion) Transcriptome analysis of PBMCs has shown the gene expression changed when co-cultured with colon cancer cell lines. The result revealed significantly up- and down-regulated genes. Overall design: Transcription profiles of PBMC (control) and PBMC co-cultured with colon cancer cell lines (treatment) were generated by deep sequencing, in triplicate for SW480 and duplicate for HT29, using Illumina HiSeq 4000.【研究目的】本研究旨在探究外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）对结肠癌细胞系的旁分泌效应。 【实验方法】采用Illumina HiSeq 4000测序平台开展深度转录组测序，获取两组样本的转录谱：对照组为单独培养的PBMCs，处理组为与结肠癌细胞系共培养的PBMCs；其中SW480共培养组设置3次生物学重复，HT29共培养组设置2次生物学重复。 【结果与结论】对PBMCs的转录组分析结果显示，当其与结肠癌细胞系共培养时，基因表达发生显著改变；本研究鉴定出一批显著上调与下调的差异表达基因。 【实验整体设计】本研究采用Illumina HiSeq 4000测序平台，通过深度测序生成对照组（单独培养的PBMCs）与处理组（与结肠癌细胞系共培养的PBMCs）的转录组图谱；其中SW480共培养样本设置3次重复，HT29共培养样本设置2次重复。