Novel germline alleles predicted by cAb_Rep database. Novel human B cell receptor germline alleles

cAb_Rep is a public database that contains 293 B cell repertoires from 108 human healthy, vaccinated, and autoimmune disease donors. The database includes a total of ~4.2 and 1.2 million curated full length V(D)J heavy and light chain transcripts respectively, which were annotated with gene origins, subtypes, mutations, and other information. By applying IgDiscover, we identified 5 novel heavy chain alleles, 2 novel lambda chain alleles, and 1 kappa chain allele, each of which were found in two or more donors.

cAb_Rep是一个公开数据库，收录了来自108名人类受试者的293组B细胞受体库（B cell repertoire），受试者涵盖健康个体、疫苗接种者及自身免疫病患者。该数据库共包含约420万条经过人工整理注释的全长V(D)J重链转录本，以及约120万条全长V(D)J轻链转录本，上述转录本均注释了基因起源、亚型、突变等相关信息。本研究通过应用IgDiscover工具，共鉴定出5个新的重链等位基因、2个新的λ链等位基因以及1个κ链等位基因，且所有新等位基因均在至少两名受试者中被检出。