Deciphering Intratumoral Molecular Heterogeneity in Clear Cell Renal Cell Carcinoma with a Radiogenomics Platform

Intratumoral heterogeneity (ITH) challenges the molecular characterization of clear cell renal cell Carcinoma (ccRCC) with percutaneous biopsies and is a confounding factor in selection of molecular-targeted versus immune-based therapy. Magnetic Resonance (MR) Imaging can noninvasively assess the spatial landscape of the entire tumor. To validate MRI for ITH assessment, we implemented a radiogenomic platform through a systematic imaging based co-localization approach for multi region tissue acquisition from single tumors. We investigated if the spatial changes in imaging can predict the molecular changes using transcriptome and histopathological correlatives. Our study confirmed imaging heterogeneity as a predictor of molecular heterogeneity in ccRCC.EGA study EGAS00001003846

肿瘤内异质性（Intratumoral heterogeneity, ITH）对经皮活检开展透明细胞肾细胞癌（clear cell renal cell carcinoma, ccRCC）的分子特征解析工作构成挑战，同时也是分子靶向治疗与免疫治疗方案选择中的混杂因素。磁共振成像（Magnetic Resonance Imaging, MR）可无创评估整个肿瘤的空间分布特征。为验证MRI在肿瘤内异质性评估中的应用价值，本研究通过基于系统性成像的共定位方法搭建了放射基因组学（radiogenomics）平台，用于从单个肿瘤中获取多区域组织样本。本研究探究了成像特征的空间变化能否通过转录组与组织病理学关联指标预测分子层面的改变，最终证实成像异质性可作为透明细胞肾细胞癌（ccRCC）分子异质性的预测因子。本研究相关数据集为EGA研究 EGAS00001003846