Super and typical enhancer switching in early mammalian development involves eRNA mediated SWI/SNF recruitment

Our data shows that the AT-hook domain of Brg1 is required for it's recruitment by an RNA dependent mechanism in distinct developmental stages. To further substantiate that the AT-hook Brg1 binds to the RNA, we purified the wild type and mutant AT-hook peptides and performed gel shift assay. Here, the gel images pertinent to the purification of the wild type and mutant AT-hook peptides and electrophoretic mobility shift assay (EMSA). The details of the experimental conditions for purification and EMSA are described in STAR Methods.

我们的数据表明，Brg1的AT-hook结构域是其在不同发育阶段通过RNA依赖机制被招募所必需的。为进一步证实Brg1的AT-hook结构域与RNA结合，我们纯化了野生型和突变型AT-hook肽段，并进行了凝胶迁移实验。此处展示的是与野生型和突变型AT-hook肽段纯化及电泳迁移率变动分析（electrophoretic mobility shift assay, EMSA）相关的凝胶图像。纯化和EMSA的实验条件细节详见STAR Methods。