HDAC8 mediated alterations in melanoma cell line gene expression. HDAC8 mediated alterations in melanoma cell line gene expression

HDAC8 expression is causative for resistance to BRAF inhibiton and increased migration in BRAF mutant melanoma cell lines. Changes in gene expression were determined upon forced HDAC8 expression in an isogenic cell line. Overall design: 1 million HDAC8 and EV expressing melanoma cells were harvested from melanoma cells grown to 70% confluence in 175 cm2 flasks. RNA was extracted and gene expression was determined to account for changes in gene expression upon HDAC8 expression.

组蛋白去乙酰化酶8 (HDAC8) 的表达与BRAF突变型黑色素瘤细胞系对BRAF抑制剂的耐药性及迁移能力提升存在因果关系。本研究通过在同基因细胞系中强制过表达HDAC8，检测其引发的基因表达变化。整体实验设计：将在175 cm²培养瓶中培养至70%细胞汇合度的黑色素瘤细胞，分别收集过表达HDAC8与空载体（EV）的细胞各1×10⁶个；提取总RNA并检测基因表达水平，以分析HDAC8过表达所导致的基因表达变化。