Cardiac-tbx20 overexpression enhances heart regeneration by promoting cardiomyocytes dedifferentiation and endocardial activation

The goal of this study is to understand the function of cardiac tbx20 during heart regeneration. By high-throughput sequencing, molecular variations of tbx20-cardiac specific inducing heart in response to heart injury compared with control hearts were demonstrated. We collected the injured heart apex tissue at 7 days post injury and sequenced the transcriptome. RNA was extracted from ventricular apical portions at 7 days post injury from control hearts and tbx20-cardiac-specific inducing hearts. Tissue from 6-8 zebrafish were collected in each control group or cardiac-tbx20-overexpressing group, three replications were carried out.

本研究旨在阐明心脏tbx20（cardiac tbx20）在心脏再生过程中的生物学功能。借助高通量测序（high-throughput sequencing）技术，本研究对比分析了心脏特异性诱导表达tbx20的斑马鱼心脏与对照心脏在应对心脏损伤时的分子表达差异。本研究于心脏损伤后7天采集损伤的心尖组织并开展转录组（transcriptome）测序；同时，从对照组与心脏特异性tbx20过表达组的斑马鱼心室心尖区域，于损伤后7天提取总RNA。每组各收集6-8条斑马鱼（zebrafish）的组织样本，并设置三次生物学重复实验。