System-wide analysis of Dhx36 function in skeletal muscle satellite cells reveals its binding with 5’UTR G4 structure to promote Gnai2 RNA translation [CLIP-seq]. System-wide analysis of Dhx36 function in skeletal muscle satellite cells reveals its binding with 5’UTR G4 structure to promote Gnai2 RNA translation [CLIP-seq]

In this study, we deciphered the functions of Dhx36 and related molecular mechanisms in muscle stem cells and muscle regeneration. We found Dhx36 was highly induced in activated muscle stem cells during regeneration induced by injury. Conditional inactivation of Dhx36 in mouse muscle stem cells caused significant impaired regeneration, which was due to the dramatically decreased cell proliferation. Dhx36 was a well-known RNA helicase for binding/unbinding DNA/RNA G-quadruplexes and it was dominantly expressed in cytoplasm of proliferating myoblast. Therefore CLIP-seq was conducted to identify the mRNAs interacting with Dhx36 in myoblast cells. Overall design: 2 Dhx36 CLIP-seq experiments performed.

本研究解析了Dhx36在肌肉干细胞及肌肉再生过程中的功能及其相关分子机制。研究发现，在损伤诱导的肌肉再生过程中，活化的肌肉干细胞内Dhx36的表达显著上调。在小鼠肌肉干细胞中条件性敲除Dhx36会导致肌肉再生功能显著受损，该表型源于细胞增殖能力的大幅下降。Dhx36是一类经典的RNA解旋酶（RNA helicase），可结合/解离DNA/RNA G-四链体，且在增殖期成肌细胞的细胞质中优势表达。因此，本研究通过CLIP-seq技术鉴定了成肌细胞中与Dhx36互作的mRNA。实验整体设计：共开展2组Dhx36 CLIP-seq实验。