Transcription patterns during wheat development

The analysis of gene expression during wheat development: Gene expression measurements were carried out on a developmental tissue series for wild-type wheat (cv. Chinese Spring) using the Affymetrix Wheat GeneChip. Thirteen tissues at defined developmental stages were chosen to match the barley (cv. Morex) tissue series of Druka et al. 2006 that used the Affymetrix Barley1 GeneChip. Three replicates of: root tissue at two different developmental stages, leaf, crown, caryopsis, anther, pistil, inflorescence, bracts, mesocotyl, endosperm, embryo and coleoptiles were hybridised. Comparisons between this wheat data and the barley dataset were performed and are available at http://contigcomp.acpfg.com.au [PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Tim Sutton. The equivalent experiment is TA3 at PLEXdb.] tissue type: germinating seed, coleoptile(3-replications); tissue type: germinating seed, root(3-replications); tissue type: germinating seed, embryo(3-replications); tissue type: seedling, root(3-replications); tissue type: seedling, crown(3-replications); tissue type: seedling, leaf(3-replications); tissue type: immature inflorescence(3-replications); tissue type: floral bracts, before anthesis(3-replications); tissue type: pistil, before anthesis(3-replications); tissue type: anthers, before anthesis(3-replications); tissue type: 3-5 DAP caryopsis(3-replications); tissue type: 22 DAP embryo(3-replications); tissue type: 22 DAP endosperm(3-replications)

小麦发育过程中的基因表达分析：本研究针对野生型普通小麦（品种中国春，cv. Chinese Spring）的发育组织系列样本，采用Affymetrix小麦基因芯片（Affymetrix Wheat GeneChip）开展基因表达量检测。研究选取13个处于明确发育阶段的组织样本，以匹配Druka等人2006年采用Affymetrix Barley1基因芯片所构建的大麦（品种Morex，cv. Morex）组织系列。针对两类不同发育阶段的根组织、叶片、分蘖节、颖果、花药、雌蕊、花序、苞片、中胚轴、胚乳、胚胎及胚芽鞘，各设置三次生物学重复并进行芯片杂交。本小麦数据集与大麦数据集的比对分析已完成，相关结果可通过http://contigcomp.acpfg.com.au 查询获取。[PLEXdb（http://www.plexdb.org）已代表原始贡献者Tim Sutton将该数据集系列提交至GEO数据库，其在PLEXdb中的对应实验编号为TA3。]各组织样本的具体分类及重复情况如下：
- 萌发种子：胚芽鞘（3次生物学重复）
- 萌发种子：胚根（3次生物学重复）
- 萌发种子：胚胎（3次生物学重复）
- 幼苗：胚根（3次生物学重复）
- 幼苗：分蘖节（3次生物学重复）
- 幼苗：叶片（3次生物学重复）
- 未成熟花序（3次生物学重复）
- 开花前花器官苞片（3次生物学重复）
- 开花前雌蕊（3次生物学重复）
- 开花前花药（3次生物学重复）
- 授粉后3-5天颖果（3次生物学重复）
- 授粉后22天胚胎（3次生物学重复）
- 授粉后22天胚乳（3次生物学重复）