JB_sinicus

Finally, a ddRAD dataset was generated, and each locus was required to be present in at least 80% of individuals (i.e., 20% missing samples per locus). To avoid linkages across sites within the same locus, one random SNP was sampled from each locus, and an unlinked SNP (USNP) dataset was ultimately generated for downstream analyses.

最终生成了一份ddRAD数据集，设定每个位点需在至少80%的个体中存在（即每个位点允许最多20%的样本缺失）。为避免同一位点内不同SNP位点间的连锁干扰，从每个位点中随机选取一个单核苷酸多态性（Single Nucleotide Polymorphism, SNP），最终得到无连锁SNP（unlinked SNP, USNP）数据集，用于后续分析。