IL-21 from high-affinity CD4 T cells drives differentiation of brain-resident CD8 T cells during persistent viral infection. IL-21 from high-affinity CD4 T cells drives differentiation of brain-resident CD8 T cells during persistent viral infection

To investigate IL-21 producing CD4 T cells, we infected IL21-VFP reporter mice with mouse polyomavirus and compared VFP+ (IL-21+) CD4 T cells to VFP- (IL-21-) CD4 T cells in the brains and spleens. Overall design: Comparative gene expression profiling analysis of RNA-seq data for VFP+ and VFP- CD4 T cells derived from the brains or spleens of IL21-VFP reporter mice at 15 day post Mouse Polyomavirus infection (dpi).

为探究产白细胞介素21（IL-21）的CD4阳性T细胞（CD4 T cells），我们使用小鼠多瘤病毒（mouse polyomavirus）感染IL21-荧光蛋白(VFP)报告基因小鼠（IL21-VFP reporter mice），并对其脑组织与脾脏中的VFP阳性（VFP+，即IL-21阳性）CD4阳性T细胞与VFP阴性（VFP-，即IL-21阴性）CD4阳性T细胞进行了比较分析。实验整体设计：对小鼠多瘤病毒感染后15天（dpi，day post infection）时，取自IL21-VFP报告基因小鼠的脑组织或脾脏中的VFP+与VFP- CD4阳性T细胞的RNA测序（RNA-seq）数据开展比较基因表达谱分析。