Linkage Map of Escherichia coli K-12, Edition 10: The Physical Map

A physical map, EcoMap10, of the now completely sequenced Escherichia coli chromosome is presented. Calculated genomic positions for the eight restriction enzymes BamHI, HindIII, EcoRI, EcoRV, BglI, KpnI, PstI, and PvuII are depicted. Both sequenced and unsequenced Kohara/Isono miniset clones are aligned to this calculated restriction map. DNA sequence searches identify the precise locations of insertion sequence elements and repetitive extragenic palindrome clusters. EcoGene10, a revised set of genes and functionally uncharacterized open reading frames (ORFs), is also depicted on EcoMap10. The complete set of unnamed ORFs in EcoGene10 are assigned provisional names beginning with the letter “y” by using a systematic nomenclature.

本研究呈现了已完成全基因组测序的大肠杆菌（Escherichia coli）染色体物理图谱EcoMap10。图谱中标注了BamHI、HindIII、EcoRI、EcoRV、BglI、KpnI、PstI及PvuII共8种限制性内切酶的预测基因组酶切位点位置。将已测序与未测序的Kohara/Isono微型克隆集克隆比对至该预测限制性酶切图谱。通过DNA序列检索，明确了插入序列元件与基因外重复回文序列簇的精准位置。经修订的基因集与功能未注释开放阅读框（ORF）集合EcoGene10，同样被标注于EcoMap10之上。EcoGene10中所有未命名开放阅读框，均通过系统命名法被赋予以字母"y"开头的临时名称。