Analysis of Escherichia coli treated and untreated with Streptomyces sp. GKU 257-1

This study is aimed to isolate marine actinomycetes from sediments from Andaman and the Gulf of Thailand. All 101 marine actinomycetes were screened for anti-biofilm activity. Streptomyces sp. GKU 257-1 showed significantly inhibited biofilm formation of E. coli. The evaluation of supernatants of anti-biofilm activity produced by Streptomyces sp. GKU 257-1 has been performed. Since the interaction between marine actinomycetes and biofilm forming bacteria has never been investigated, proteomic analysis has been used to identify whole cell proteins involved in anti–biofilm activity. Understanding the interaction at molecular level will lead to sustainably use for anti-biofilm producing marine actinomycetes in pharmaceutical and medicinal applications in the future.

本研究旨在从安达曼海与泰国湾的沉积物中分离海洋放线菌（marine actinomycetes）。本研究对全部101株海洋放线菌开展了抗生物膜活性（anti-biofilm activity）筛选，结果显示链霉菌属（Streptomyces）菌株GKU 257-1可显著抑制大肠杆菌（Escherichia coli，缩写E. coli）的生物膜形成。本研究已完成对链霉菌属菌株GKU 257-1所产抗生物膜活性上清液的活性评价。鉴于目前尚未有关于海洋放线菌与生物膜形成细菌之间相互作用的相关研究，本研究采用蛋白质组学分析（proteomic analysis）技术，以鉴定参与抗生物膜活性的全细胞蛋白质。在分子层面解析该相互作用，将为未来抗生物膜海洋放线菌在制药与医药领域的可持续应用提供理论支撑。