Nardostachys jatamansi Transcriptome or Gene expression

Nardostachys jatamansi is a Himalayan medicinal plant, known for its excellent antioxidant and metabolic activity. Two different gold nanoparticles with distinct charge on the surface (positive and negative) modulate growth, photosynthetic activity and antioxidant potential of the plant. To decipher the transcripts and pathways underlying gold nano elicitation of N. jatamansi, transcriptome of in-vitro shoots subjected to control (NC), negative charged AuNPs (NT1) and positive charged AuNPs (NT2) was sequenced. The study generated total 217.1 million clean reads that identified 104, 1255, and 660 significant differentially expressed genes (DEGs) in NC vs NT1, NC vs NT2 and NT1 vs NT2 libraries, respectively. The Gene ontology, KEGG pathway, DEGs and transcription factors were found to be associated with plant growth, antioxidant activity, hormone metabolism, cellular metabolism and abiotic stress resistance. Altogether, the study provides insights into the transcriptomic response of N. jatamansi during differentially charged gold nanoparticles elicitation and concluded that CTAB coated (positive charged) gold nanoparticles are more efficient than citrate coated (negative charged) gold nanoparticles.

匙叶甘松（Nardostachys jatamansi）是一种喜马拉雅特色药用植物，以其优异的抗氧化活性与代谢活性著称。两种表面电荷属性截然不同的金纳米颗粒（gold nanoparticles, AuNPs），可调控该植物的生长、光合活性与抗氧化潜能。为解析甘松经金纳米颗粒诱导后的转录本与核心分子通路机制，本研究对经三种处理的离体培养芽苗进行转录组测序：对照组（NC）、带负电金纳米颗粒处理组（NT1）以及带正电金纳米颗粒处理组（NT2）。本研究共获得2.171亿条清洁读段，分别在NC vs NT1、NC vs NT2、NT1 vs NT2的文库中，鉴定出104、1255与660个显著差异表达基因（differentially expressed genes, DEGs）。基因本体（Gene Ontology, GO）注释、京都基因与基因组百科全书（KEGG）通路富集分析、差异表达基因及转录因子的相关分析结果显示，上述分子事件与植物生长、抗氧化活性、激素代谢、细胞代谢及非生物胁迫抗性密切相关。综上，本研究揭示了甘松在不同电荷金纳米颗粒诱导处理下的转录组响应特征，并证实十六烷基三甲基溴化铵（CTAB）包被的带正电金纳米颗粒，其诱导效果优于柠檬酸盐包被的带负电金纳米颗粒。