salivary microbiome and metabolites associated with immunotherapy efficacy in lung cancer

Lung cancer is a leading cause of cancer mortality, with NSCLC comprising the majority of cases. Despite the advent of immune checkpoint inhibitors(ICIs), a significant number of patients fail to achieve durable respond, highlighting the need to understand the factors influencing treatment efficacy.Salivasamples and tumor samplesfrom 20 NSCLC patientswere collected. The salivary microbiota was profiled using mNGS and the metabolites were analyzed via LC/MS to identify the correlationsamong bacteria, metabolitesand immunotheropy responses.

肺癌是癌症死亡的首要原因，其中非小细胞肺癌（NSCLC）占病例的大多数。尽管免疫检查点抑制剂（ICIs）已问世，但仍有相当数量的患者未能获得持久应答，这凸显了理解影响治疗疗效因素的必要性。本研究收集了20例NSCLC患者的唾液样本和肿瘤样本。通过宏基因组下一代测序（mNGS）对唾液微生物群进行特征分析，并通过液相色谱-质谱联用技术（LC/MS）分析代谢物，以确定细菌、代谢物与免疫治疗应答之间的相关性。