Effects of PI3K inhibitors on extended lamellipodia and peripheral ruffles in Rac1Q61L expressing PC-3 cells.

Thirty mCitrine-Rac1Q61L expressing cells in each group were subjected to quantitative image analysis. After 30 min of the indicated drug treatments, the cells were fixed with 4% paraformaldehyde, rinsed in PBS and observed by fluorescence microscopy.
aThe maximum diameter of each cell was measured using MetaMorph.
bThe number of peripheral ruffles per each cell was counted. Data are the means ± SE (n = 30).
*p<0.01 compared to the control.

每组各30株表达mCitrine-Rac1Q61L的细胞被用于定量图像分析。经指定药物处理30分钟后，将细胞以4%多聚甲醛固定，经磷酸盐缓冲液（PBS）漂洗后，通过荧光显微镜观察。
a 采用MetaMorph软件测定每株细胞的最大直径。
b 计数每株细胞的周边褶皱数目。实验数据以平均值±标准误（Standard Error，SE）表示，样本量n=30。
* 与对照组相比，p<0.01。