Transcriptome analysis of AvrPi9-interacting protein 1 (ANIP1) knock-out plants. Oryza sativa Japonica Group

To understand how ANIP1 participates in immune response, we monitored the whole-transcriptome of wild type (Nipponbare) and ANIP1-KO (anip1) plants. Total RNA was isolated from 3-week-old seedlings with three repeats and subjected to high throughput sequencing using Illumina NovaSeq 6000 PE-150. Magnaporthe oryzae (Mo) treatment was performed by inoculating the seedlings with the compatible isolate RB22 for 48 h.

为阐明ANIP1参与免疫应答的分子机制，我们对野生型（日本晴，Nipponbare）及ANIP1基因敲除（anip1）植株开展全转录组分析。我们从3周龄幼苗中提取总RNA，设置三次生物学重复，并采用Illumina NovaSeq 6000 PE-150平台进行高通量测序。我们采用亲和性菌株RB22接种幼苗，完成了48小时的稻瘟病菌（Magnaporthe oryzae，Mo）处理。