Transcriptome analysis reveals the molecular mechanism of differences in growth between photoautotrophy and heterotrophy in Chlamydomonas reinhardtii

In the transcriptome analysis, the CC-125 strain was cultured in photoautotrophy for 12 hours, then collected 2×10^7 algal cells (for the P12h group). The remaining CC-125 cultures were rinsed three times with TAP medium and immediately transferred to a conical flask containing TAP medium for further heterotrophy cultivation (as mentioned above) for 12 hours. We again collected 2ⅹ10^7 algal cells (for the H12h group).

在转录组分析中，将CC-125菌株以光合自养（photoautotrophy）方式培养12小时，随后收集2×10^7个藻类细胞，记为P12h组。将剩余的CC-125培养液用TAP培养基冲洗三次，随后立即转移至装有TAP培养基的锥形瓶中，继续进行异养培养（heterotrophy cultivation，如前所述）12小时，再次收集2×10^7个藻类细胞，记为H12h组。