pHlim source data

Changes in sub-cellular pH play a key role in metabolism, cell growth, membrane transport, and can also be exploited to control cargo release from therapeutic delivery systems. Most methods to measure pH rely on intensity changes of pH sensitive fluorophores, however these measurements are hampered by high uncertainty in the inferred pH and the need for multiple fluorophores. To address this, we have developed a method to accurately quantify sub-cellular pH in individual vesicles using fluorescent lifetime imaging microscopy (pHLIM). This project contains all the source data for the corresponding Nature Communications article.

亚细胞pH（sub-cellular pH）的变化在代谢、细胞生长及膜运输过程中发挥关键作用，同时还可被用于调控治疗递送系统中的货物释放。目前多数pH检测方法均依赖于pH敏感荧光团（pH sensitive fluorophores）的强度变化，但这类检测方法受限于推算pH值时的较高不确定性，且需要使用多种荧光团。为解决这一问题，我们开发了一种借助荧光寿命成像显微镜（fluorescent lifetime imaging microscopy，pHLIM）精准定量单个囊泡内亚细胞pH的方法。本数据集包含对应《自然-通讯》（Nature Communications）论文的全部源数据。