Gene expression during early postnatal development of the small intestine. Mus musculus

It was the purpose to analyse the changes in gene expression which occur in the mouse small intestine from the pre-weaning to the post-weaning stage. The gene expression was accordingly followed from postnatal day 4 to postnatal day 32. Keywords: Time-course Overall design: 6 time-points were defined corresponding to postnatal day 4, 7, 9, 11, 24 and 32. Ileal samples were taken from individual mice at each time-point. All layers of the small intestine were included in the sample. Three samples from individual mice were analysed by hybridization to Affymetrix GeneChips.

本研究旨在分析小鼠小肠从断奶前到断奶后阶段的基因表达变化。据此，我们对小鼠出生后第4天至第32天的基因表达进行了追踪。关键词：时间序列（Time-course）；实验设计：共设置6个时间点，分别对应出生后第4、7、9、11、24和32天。在每个时间点，均从单只小鼠体内采集回肠样本，样本涵盖小肠的全部组织层。通过Affymetrix基因芯片（Affymetrix GeneChips）杂交技术，对来自3只独立小鼠的样本完成了检测分析。