Homo sapiens Raw sequence reads. Homo sapiens

The acetylation modification of lysine 9 on histone H3 and lysine 27 on histone H3 is an important mechanism of transcriptional regulation. In this study, CUT&TAG sequencing was performed on cell samples of the HuCCT1 cell line with targeted shRNA against the SLC16A3 gene sequence and on empty vector controls, with the aim of analyzing the levels of H3K9ac and H3K27ac across the genome. The results showed that the knockdown of SLC16A3 expression resulted in a significant increase in both H3K9ac and H3K27ac levels across a wide range of genes, leading to an upregulation of acetylation levels at TSS regions for a series of important tumor suppressor genes. Additionally, transcriptome sequencing was conducted on these same samples, and its results were compared and intersected with those from CUT&TAG to verify that the upregulation in acetylation levels at TSS regions corresponded to an increase in expression level for these tumor suppressor genes at the transcriptional level.

组蛋白H3第9位赖氨酸与组蛋白H3第27位赖氨酸的乙酰化修饰，是转录调控的重要机制。本研究针对SLC16A3基因序列设计靶向短发夹RNA（short hairpin RNA, shRNA），并以空载体作为对照，对HuCCT1细胞系的细胞样本开展CUT&TAG测序（CUT&TAG Sequencing），旨在分析全基因组范围内H3K9乙酰化（H3K9ac）与H3K27乙酰化（H3K27ac）的修饰水平。结果显示，敲低SLC16A3基因的表达会使大量基因的H3K9ac与H3K27ac修饰水平显著升高，导致一系列重要抑癌基因的转录起始位点（Transcription Start Site, TSS）区域乙酰化水平上调。此外，本研究对上述样本开展了转录组测序（Transcriptome Sequencing），并将其结果与CUT&TAG测序结果进行对比与交叉分析，以验证TSS区域乙酰化水平上调与这些抑癌基因在转录水平的表达量升高具有对应关系。