Table4_Causal analysis of the gut microbiota in differentiated thyroid carcinoma: a two-sample Mendelian randomization study.DOCX

Objective: Numerous studies have highlighted an association between the gut microbiota (GM) and thyroid tumors. Employing Mendelian randomization methodology, we seek to elucidate the causal link between the gut microbiota and thyroid neoplasms.
Methods: We procured data from the Mibiogen database encompassing 211 distinct gut microbiota taxa, alongside extensive genome-wide association studies (GWAS) summary data for differentiated thyroid carcinoma (DTC). Our principal analytical approach involved the application of the Inverse-Variance Weighted method (IVW) within the framework of Mendelian randomization. Simultaneously, we conducted sensitivity analyses to assess result heterogeneity, horizontal pleiotropy, and outcome stability.
Results: IVW analysis revealed a dual role of the GM in thyroid carcinoma. The phylum Actinobacteria (OR, 0.249 [95% CI, 0.121–0.515]; p < 0.001) was associated with a decreased risk of DTC. Conversely, the genus Ruminiclostridium9 (OR, 11.276 [95% CI, 4.406–28.860]; p < 0.001), class Mollicutes (OR, 5.902 [95% CI, 1.768–19.699]; p = 0.004), genus RuminococcaceaeUCG004 (OR, 3.831 [95% CI, 1.516–9.683]; p = 0.005), genus Paraprevotella (OR, 3.536 [95% CI, 1.330–9.401]; p = 0.011), and phylum Tenericutes (OR, 5.902 [95% CI, 1.768–19.699]; p = 0.004) were associated with an increased risk of DTC.
Conclusion: Our findings underscore that the presence of genus Ruminiclostridium9, class Mollicutes, genus RuminococcaceaeUCG004, genus Paraprevotella, and phylum Tenericutes is associated with an elevated risk of DTC, whereas the presence of the phylum Actinobacteria is linked to a decreased risk. These discoveries enhance our comprehension of the relationship between the GM and DTC.

研究目的：诸多研究已证实肠道菌群（gut microbiota, GM）与甲状腺肿瘤存在关联。本研究拟采用孟德尔随机化（Mendelian randomization）方法，阐明肠道菌群与甲状腺肿瘤之间的因果联系。
研究方法：本研究从Mibiogen数据库获取了涵盖211种不同肠道菌群分类群的数据，同时获取了分化型甲状腺癌（differentiated thyroid carcinoma, DTC）的大规模全基因组关联研究（genome-wide association studies, GWAS）汇总数据。本研究的核心分析方法为在孟德尔随机化框架下应用逆方差加权法（Inverse-Variance Weighted method, IVW）。同时开展敏感性分析，以评估研究结果的异质性、水平多效性及结局稳定性。
研究结果：逆方差加权法分析显示，肠道菌群在甲状腺癌中发挥双重作用。放线菌门（Actinobacteria）的比值比（odds ratio, OR）为0.249 [95%置信区间（confidence interval, CI）：0.121~0.515]，p < 0.001，其与分化型甲状腺癌的发病风险降低相关。反之，瘤胃梭菌属9（Ruminiclostridium9）的OR值为11.276 [95%CI：4.406~28.860]，p < 0.001；柔膜菌纲（Mollicutes）的OR值为5.902 [95%CI：1.768~19.699]，p=0.004；瘤胃球菌科UCG004属（RuminococcaceaeUCG004）的OR值为3.831 [95%CI：1.516~9.683]，p=0.005；副普雷沃氏菌属（Paraprevotella）的OR值为3.536 [95%CI：1.330~9.401]，p=0.011；柔膜菌门（Tenericutes）的OR值为5.902 [95%CI：1.768~19.699]，p=0.004，上述分类群均与分化型甲状腺癌的发病风险升高相关。
研究结论：本研究结果表明，瘤胃梭菌属9、柔膜菌纲、瘤胃球菌科UCG004属、副普雷沃氏菌属以及柔膜菌门的存在与分化型甲状腺癌的发病风险升高相关，而放线菌门的存在则与该病的发病风险降低相关。上述发现加深了我们对肠道菌群与分化型甲状腺癌之间关联的理解。