Next generation sequencing and de novo transcriptome analysis of Papilio clytia and Atrophaneura mencius larvae upon lipopolysaccharide challenge

Gene expression profiling was performed by next generation sequencing strategy to explore the transcriptional changes of Papilio clytia and Atrophaneura mencius larvae in response to LPS challenge The fatbody tissues of Papilio clytia and Atrophaneura mencius larvae were collected for RNA isolation and high-throughput sequencing

本研究采用下一代测序（next generation sequencing, NGS）策略开展基因表达谱分析，以探究斑凤蝶（Papilio clytia）与麝凤蝶（Atrophaneura mencius）幼虫在脂多糖（LPS）刺激下的转录组变化。研究人员采集了该两种幼虫的脂肪体组织，用于RNA提取及高通量测序。