Primer sequences and predicted amplicon sizes.

BRAF and MAP2K1 target regions are listed with the corresponding primer sequences and 5’ fluorophore tag. The underlined sequence represents the 17 nucleotides that allow size discrimination between the BRAF V595 wild-type and mutant alleles. The lower case nucleotide represents the additional sequence mismatch introduced into the forward primer for the mutant allele to prevent spurious binding with the wild-type allele. The last two columns indicate the expected amplicon size obtained from wild-type alleles, and the observed amplicon sizes from the study cohort. (XLSX)

本数据集列出了BRAF与MAP2K1的靶标区域，及其对应的引物序列与5'荧光基团标签（5’ fluorophore tag）。下划线序列为17个核苷酸，可实现BRAF V595野生型与突变型等位基因的长度鉴别。小写核苷酸为引入至突变型等位基因正向引物中的额外序列错配，用于避免与野生型等位基因发生非特异性结合。最后两列分别标注了野生型等位基因的预期扩增子（amplicon）长度，以及本研究队列中实测得到的扩增子长度。(XLSX)