Rapid and affordable genome-wide bisulfite DNA sequencing by XmaI-RRBS. Homo sapiens

We describe XmaI-RRBS method for rapid and affordable genome-wide DNA methylation analysis, with library preparation taking only four days and sequencing possible within four hours. Small sizes of the XmaI-RRBS libraries allow their multiplexing and sequencing on the benchtop high-throughput machines. Described here is the first RRBS protocol validated for the Ion Torrent Personal Genome Machine. Overall design: DNA from MCF7 cell line and 6 normal breast samples (total 7 samples) were subjected to reduced representation bisulfite sequencing analysis (XmaI-RRBS) by using Ion Torrent platform.

本研究介绍了一种可实现快速且经济的全基因组DNA甲基化分析的XmaI限制性代表性亚硫酸氢盐测序（XmaI-RRBS）方法，其文库制备仅需4天，测序可在4小时内完成。该方法制备的XmaI-RRBS文库体量小巧，可实现多重测序，并适配台式高通量测序仪运行。本研究首次报道了一款经验证可适配Ion Torrent个人基因组测序仪（Ion Torrent Personal Genome Machine）的RRBS实验方案。
实验设计：以MCF7细胞系及6份正常乳腺组织样本（共计7份样本）的DNA为材料，采用Ion Torrent测序平台开展XmaI限制性代表性亚硫酸氢盐测序（XmaI-RRBS）分析。