The role of Erg in B cell development (RNA-Seq)

RNA-seq of pre-proB, proB and preB populations from Ergfl/fl bone marrow, pre-proB cell population from Rag1CreT/+;ErgΔ/Δ bone marrow Two biological replicates of each primary B-cell progenitor population were purified from bone marrow of the specified genotypes using flow cytometry sorting. Transcriptomic profiling was undertaken on purified populations.

本数据集针对来自Ergfl/fl小鼠骨髓的前前B细胞（pre-proB）、前B细胞（proB）与未成熟前B细胞（preB）群，以及来自Rag1CreT/+;ErgΔ/Δ小鼠骨髓的前前B细胞群开展RNA测序（RNA-seq）。每个原代B细胞祖细胞群均设置2个生物学重复，通过流式细胞分选从指定基因型小鼠的骨髓中纯化获得，随后对纯化后的细胞群进行转录组谱分析。