Additional file 1 of A tomato NAC transcription factor, SlNAP1, directly regulates gibberellin-dependent fruit ripening

Additional file 1: Figure S1. SlNAP1 protein belongs to the NAC transcription factor (TF) family. Figure S2. A phylogenetic tree showing the evolutionary relationships of the subgenus families, including the known genes involved in tomato fruit ripening. Figure S3. Fruit phenotype and SlNAP1 gene expression of TRV and TRV-SlNAP1. Figure S4. Ethylene content and the expression levels of ethylene synthesis-related genes ACC oxidase 1 (SlACO1), ACC oxidase 1 (SlACO3), ACC synthase 2 (SlACS2), and ACC synthase 4 (SlACS4) in wild-type (WT), slnap1-5, and slnap1-16 fruits at mature green (MG), breaker (Br), 4 days after the breaker (Br + 4), and 7 days after the breaker (Br + 7) stages. ACC,1-aminocyclopropyl 1-carboxylic acid. Figure S5. Chlorophyll (Chl) and carotenoid (Car) content in wild-type (WT), slnap1-5, and slnap1-16 fruits. Figure S6. The expression levels of chlorophyll (Chl) degradation-related genes non-yellow coloring1 (SlNYC1), stay green 1 (SlSGR1), pheophide a oxygenase (SlPAO), pheophytinase (SlPPH) and red chlorophyll catabolite reductase (SlRCCR) in wild-type (WT), slnap1-5, and slnap1-16 fruits at mature green (MG) stage. Figure S7. The expression levels of carotenoid (Car) synthesis-related genes phytoene synthase 1 (SlPSY1; A), phytoene synthase 2 (SlPSY2; B), phytoene synthase 3 (SlPSY3; C), copalyl diphosphate synthases (SlCPPS; D), isoprenyl diphosphate synthases (SlIDS; E), lycopene ß-cyclase 1 (SlLCYB1; F), lycopene ß-cyclase 2 (SlLCYB2; G), lycopene δ-cyclase (SlLCYE; H), β-carotene hydroxylase 1 (SlCYHB1; I), β-carotene hydroxylase 2 (SlCYHB2; J), violaxanthin deepoxidase (SlVED; K), zeaxanthin epoxidase (SlZFP; L) in wild-type (WT), slnap1-5, and slnap1-16 fruits at breaker (Br), 4 days after the breaker (Br + 4), and 7 days after the breaker (Br + 7) stages. Figure S8. The effect of gibberellin (GA) on the interaction between SlNAP1 and SlGID1. Table S1. Primers used for vector construction. Table S2. Primers used for quantitative real-time PCR (qRT-PCR). Table S3. Primers used for target site mutation analysis.

附加文件1：
图S1：SlNAP1蛋白属于NAC转录因子（transcription factor, TF）家族。
图S2：系统发育树展示了相关亚属家族的进化关系，涵盖已知参与番茄果实成熟过程的基因。
图S3：TRV与TRV-SlNAP1处理组的果实表型及SlNAP1基因表达水平。
图S4：野生型（wild-type, WT）、slnap1-5及slnap1-16番茄果实于绿熟期（mature green, MG）、破色期（breaker, Br）、破色后4天（4 days after the breaker, Br+4）以及破色后7天（7 days after the breaker, Br+7）的乙烯含量，以及乙烯合成相关基因ACC氧化酶1（ACC oxidase 1, SlACO1）、ACC氧化酶3（SlACO3）、ACC合酶2（ACC synthase 2, SlACS2）与ACC合酶4（SlACS4）的表达水平。注：ACC即1-氨基环丙烷-1-羧酸（1-aminocyclopropyl 1-carboxylic acid）。
图S5：野生型（WT）、slnap1-5及slnap1-16番茄果实中的叶绿素（chlorophyll, Chl）与类胡萝卜素（carotenoid, Car）含量。
图S6：野生型（WT）、slnap1-5及slnap1-16番茄果实于绿熟期（MG）时，叶绿素降解相关基因非黄化1（non-yellow coloring1, SlNYC1）、持绿1（stay green 1, SlSGR1）、脱镁叶绿酸a加氧酶（pheophide a oxygenase, SlPAO）、脱植基叶绿素酶（pheophytinase, SlPPH）以及红色叶绿素代谢物还原酶（red chlorophyll catabolite reductase, SlRCCR）的表达水平。
图S7：野生型（WT）、slnap1-5及slnap1-16番茄果实于破色期（Br）、破色后4天（Br+4）以及破色后7天（Br+7）时，类胡萝卜素合成相关基因八氢番茄红素合酶1（phytoene synthase 1, SlPSY1；A）、八氢番茄红素合酶2（SlPSY2；B）、八氢番茄红素合酶3（SlPSY3；C）、巴豆基焦磷酸合酶（copalyl diphosphate synthases, SlCPPS；D）、异戊烯基二磷酸合酶（isoprenyl diphosphate synthases, SlIDS；E）、番茄红素β-环化酶1（lycopene β-cyclase 1, SlLCYB1；F）、番茄红素β-环化酶2（SlLCYB2；G）、番茄红素δ-环化酶（lycopene δ-cyclase, SlLCYE；H）、β-胡萝卜素羟化酶1（β-carotene hydroxylase 1, SlCYHB1；I）、β-胡萝卜素羟化酶2（SlCYHB2；J）、紫黄质脱环氧酶（violaxanthin deepoxidase, SlVED；K）以及玉米黄质环氧酶（zeaxanthin epoxidase, SlZFP；L）的表达水平。
图S8：赤霉素（gibberellin, GA）对SlNAP1与SlGID1蛋白互作的影响。
表S1：用于载体构建的引物序列。
表S2：用于实时荧光定量PCR（quantitative real-time PCR, qRT-PCR）的引物序列。
表S3：用于靶位点突变分析的引物序列。